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Literature summary extracted from

  • Li, M.; Martinez, S.; Hausinger, R.P.; Emerson, J.P.
    Thermodynamics of iron(II) and substrate binding to the ethylene-forming enzyme (2018), Biochemistry, 57, 5696-5705 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.19 His-tagged form of enzyme is generated in Escherichia coli BL21 Gold (DE3) cells Pseudomonas syringae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.12.19 Fe2+ the nonheme iron(II) oxygenase catalyzes the oxidative decarboxylation of 2-oxoglutarate to succinate and CO2 to generate a highly reactive iron species that hydroxylates a specific alkane C-H bond, in this case targeting L-arginine (Arg) for hydroxylation Pseudomonas syringae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.19 2-oxoglutarate + O2 Pseudomonas syringae
-
 ethene + 3 CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.19 Pseudomonas syringae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.19 2-oxoglutarate + O2
-
 Pseudomonas syringae ethene + 3 CO2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.19 EFE
-
 Pseudomonas syringae
1.13.12.19 ethylene-forming enzyme
-
 Pseudomonas syringae